Protein scaffold of a heart12/25/2022 Specifically, collagen type III provides the elasticity of the matrix while collagen type I contributes to the overall rigidity of the myocardial tissue. Collagen type I and III are the major fibrous protein components of the myocardium ECM, which play an essential role in structural integrity and mechanical robustness of the tissue construct. The extracellular matrix (ECM) of the myocardial tissue exhibits well-defined three dimensional (3D) fibrillar architecture, which provides the essential signaling cues for alignment, organization and synchronized beating of cardiac cells. Overall, our study suggests that the aligned nanofibrous PGS:gelatin scaffold support cardiac cell organization, phenotype and contraction and could potentially be used to develop clinically relevant constructs for cardiac tissue engineering. % PGS, induced optimal synchronous contractions of CMs while significantly enhanced cellular alignment. Notably, aligned nanofibrous scaffold, consisting of 33 wt. Furthermore, we studied attachment, proliferation, differentiation and alignment of neonatal rat cardiac fibroblast cells (CFs) as well as protein expression, alignment, and contractile function of cardiomyocyte (CMs) on PGS:gelatin scaffolds with variable amount of PGS. Our findings demonstrated that through incorporation of PGS, it is possible to create nanofibrous scaffolds with well-defined anisotropy that mimics the left ventricular myocardium architecture. In this study, we utilized an electrospinning approach to fabricate elastomeric biodegradable poly(glycerol-sebacate) (PGS):gelatin scaffolds with a wide range of chemical composition, stiffness and anisotropy. However previous attempts have not been able to simultaneously recapitulate chemical, mechanical, and structural properties of the myocardial extracellular matrix (ECM). A number of approaches have used engineered scaffolds to mimic the architecture of the native myocardium tissue and precisely regulate cardiac cell functions. A significant challenge in cardiac tissue engineering is the development of biomimetic grafts that can potentially promote myocardial repair and regeneration.
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